Simultaneous Equations in Excel for UV/vis Analysis (Matrix Method)
We want to solve this problem using Excel. There are two ways to approach it (easily). In this second method, we will look at the matrix method: (The first method is to use circular references)
Two substances, A and B, absorb light according to the Beer-Lambert law A = ecL where e is the extinction coefficient, c is the concentration of the absorbing substance and L is the path length (0.1 dm). If both species are in solution and the absorbance at 300 nm is measured to be 3.5 and the absorbance at 500 nm is 2.3, use simultaneous equations to calculate the concentrations of A and B in the mixed solution. The extinction coefficients, e are:
* At 300 nm, e(A) = 80; e(B) = 150 units
* At 500 nm, e(A) = 52, e(B) = 100 units.













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